Abstract
BACKGROUND: Defensins are antimicrobial peptides and uniformly spans the entire sperm surface and is not exclusive to a specific domain. Goat β-defensin-1 helps in initiation of motility and capacitation of sperm. OBJECTIVE: To know the status of β-defensin-1 in blood, semen and its effect on post thaw fertility gene expression in Indian goat breeds. MATERIALS AND METHODS: Semen was extended and divided for estimation of β-defensin-1 and cryopreserved having different concentrations of β-defensin-1. RESULTS: Bet defensin-1 concentration (pg/mL) in neat semen, sperm pellet and seminal plasma was significantly higher (P<0.05) in goat breed Barbari followed by Jamunapari and Jakhrana. Beta defensing-1 was also high in Jakhrana blood followed by Barbari and Jamunapari. The post thaw motility, live sperm, acrosome intactness and hypo osmotic swelled sperms were significantly higher (P< 0.05) with 10 ng/mL β-defensin in the semen dilutor. CONCLUSION: Beta defensin (10 ng/mL) in semen dilutor may be used as immuno-modulator to get better post thaw quality suitable for artificial insemination.
| Original language | English |
|---|---|
| Pages (from-to) | 137-145 |
| Number of pages | 9 |
| Journal | Cryo-Letters |
| Volume | 42 |
| Issue number | 3 |
| Publication status | Published - May 2021 |
| Externally published | Yes |
Keywords
- artificial insemination
- beta defensin
- gene expression
- immunomodulatory
- semen cryopreservation