Structural studies of the cell wall polysaccharide from Lactococcus lactis UC509.9

Evgeny Vinogradov; Irina Sadovskaya; Thierry Grard; James Murphy; Jennifer Mahony; Marie Pierre Chapot-Chartier; Douwe van Sinderen

doi:10.1016/j.carres.2018.03.011

Structural studies of the cell wall polysaccharide from Lactococcus lactis UC509.9

Evgeny Vinogradov
, Irina Sadovskaya
, Thierry Grard
, James Murphy
, Jennifer Mahony
, Marie Pierre Chapot-Chartier
, Douwe van Sinderen

Research output: Contribution to journal › Article › peer-review

Abstract

Lactococcus lactis is the most widely utilised starter bacterial species in dairy fermentations. The L. lactis cell envelope contains polysaccharides, which, among other known functions, serve as bacteriophage receptors. Our previous studies have highlighted the structural diversity of these so-called cell wall polysaccharides (CWPSs) among L. lactis strains that could account for the narrow host range of most lactococcal bacteriophages. In the present work, we studied the CWPS of L. lactis strain UC509.9, an Irish dairy starter strain that is host to the temperate and well-characterized P335-type phage Tuc2009. The UC509.9 CWPS structure was analyzed by methylation, deacetylation/deamination, Smith degradation and 2D NMR spectroscopy. The CWPS consists of a linear backbone composed of a tetrasaccharide repeat unit, partially substituted with a branched phosphorylated oligosaccharide having a common trisaccharide and three non-stoichiometric substitutions.

Original language	English
Pages (from-to)	25-31
Number of pages	7
Journal	Carbohydrate Research
Volume	461
DOIs	https://doi.org/10.1016/j.carres.2018.03.011
Publication status	Published - 22 May 2018

Keywords

Cell-wall polysaccharide
Lactococcus lactis
NMR spectroscopy
Structure

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10.1016/j.carres.2018.03.011

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title = "Structural studies of the cell wall polysaccharide from Lactococcus lactis UC509.9",

abstract = "Lactococcus lactis is the most widely utilised starter bacterial species in dairy fermentations. The L. lactis cell envelope contains polysaccharides, which, among other known functions, serve as bacteriophage receptors. Our previous studies have highlighted the structural diversity of these so-called cell wall polysaccharides (CWPSs) among L. lactis strains that could account for the narrow host range of most lactococcal bacteriophages. In the present work, we studied the CWPS of L. lactis strain UC509.9, an Irish dairy starter strain that is host to the temperate and well-characterized P335-type phage Tuc2009. The UC509.9 CWPS structure was analyzed by methylation, deacetylation/deamination, Smith degradation and 2D NMR spectroscopy. The CWPS consists of a linear backbone composed of a tetrasaccharide repeat unit, partially substituted with a branched phosphorylated oligosaccharide having a common trisaccharide and three non-stoichiometric substitutions.",

keywords = "Cell-wall polysaccharide, Lactococcus lactis, NMR spectroscopy, Structure",

author = "Evgeny Vinogradov and Irina Sadovskaya and Thierry Grard and James Murphy and Jennifer Mahony and Chapot-Chartier, \{Marie Pierre\} and \{van Sinderen\}, Douwe",

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doi = "10.1016/j.carres.2018.03.011",

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T1 - Structural studies of the cell wall polysaccharide from Lactococcus lactis UC509.9

AU - Vinogradov, Evgeny

AU - Sadovskaya, Irina

AU - Grard, Thierry

AU - Murphy, James

AU - Mahony, Jennifer

AU - Chapot-Chartier, Marie Pierre

AU - van Sinderen, Douwe

PY - 2018/5/22

Y1 - 2018/5/22

N2 - Lactococcus lactis is the most widely utilised starter bacterial species in dairy fermentations. The L. lactis cell envelope contains polysaccharides, which, among other known functions, serve as bacteriophage receptors. Our previous studies have highlighted the structural diversity of these so-called cell wall polysaccharides (CWPSs) among L. lactis strains that could account for the narrow host range of most lactococcal bacteriophages. In the present work, we studied the CWPS of L. lactis strain UC509.9, an Irish dairy starter strain that is host to the temperate and well-characterized P335-type phage Tuc2009. The UC509.9 CWPS structure was analyzed by methylation, deacetylation/deamination, Smith degradation and 2D NMR spectroscopy. The CWPS consists of a linear backbone composed of a tetrasaccharide repeat unit, partially substituted with a branched phosphorylated oligosaccharide having a common trisaccharide and three non-stoichiometric substitutions.

AB - Lactococcus lactis is the most widely utilised starter bacterial species in dairy fermentations. The L. lactis cell envelope contains polysaccharides, which, among other known functions, serve as bacteriophage receptors. Our previous studies have highlighted the structural diversity of these so-called cell wall polysaccharides (CWPSs) among L. lactis strains that could account for the narrow host range of most lactococcal bacteriophages. In the present work, we studied the CWPS of L. lactis strain UC509.9, an Irish dairy starter strain that is host to the temperate and well-characterized P335-type phage Tuc2009. The UC509.9 CWPS structure was analyzed by methylation, deacetylation/deamination, Smith degradation and 2D NMR spectroscopy. The CWPS consists of a linear backbone composed of a tetrasaccharide repeat unit, partially substituted with a branched phosphorylated oligosaccharide having a common trisaccharide and three non-stoichiometric substitutions.

KW - Cell-wall polysaccharide

KW - Lactococcus lactis

KW - NMR spectroscopy

KW - Structure

UR - https://www.scopus.com/pages/publications/85044160693

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DO - 10.1016/j.carres.2018.03.011

M3 - Article

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AN - SCOPUS:85044160693

SN - 0008-6215

VL - 461

SP - 25

EP - 31

JO - Carbohydrate Research

JF - Carbohydrate Research

ER -

Structural studies of the cell wall polysaccharide from Lactococcus lactis UC509.9

Abstract

Keywords

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