Structure of the human retinoic acid receptor β1 gene

André Toulouse; Johane Morin; Manon Pelletier; W. E.C. Bradley

doi:10.1016/S0167-4781(96)00126-1

Structure of the human retinoic acid receptor β1 gene

André Toulouse
, Johane Morin
, Manon Pelletier
, W. E.C. Bradley

University of Montreal

Research output: Contribution to journal › Article › peer-review

Abstract

We report the isolation and characterization of genomic sequences coding for the 5' end of human retinoic acid receptor β1, a fetal RAR isoform which is also expressed in small cell lung cancers. Primer extension analysis revealed a principal transcription start site with a secondary site 23 bp further upstream in both SCLC and fetal tissues. The sequences isolated were CpG-rich between -60 and the 3' end of the first exon but there were no features like a TATA-box or an Inr element. In several important aspects, the human RARβ1 differs from its mouse counterpart. The 5'-UTR is 823 bp long and is encoded by four exons (vs two in the mouse giving a 477 bp UTR). The 5' end of the mouse cDNA has been mapped to a 67 bp CT repeat which is absent in our sequences. We were also unable to find evidence for a human isoform RARβ3 which is widely expressed in mouse tissues.

Original language	English
Pages (from-to)	1-4
Number of pages	4
Journal	Biochimica et Biophysica Acta - Gene Structure and Expression
Volume	1309
Issue number	1-2
DOIs	https://doi.org/10.1016/S0167-4781(96)00126-1
Publication status	Published - 11 Nov 1996
Externally published	Yes

Keywords

5'-Untranslated region
Gene structure
Retinoic acid receptor

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0167-4781(96)00126-1

Cite this

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title = "Structure of the human retinoic acid receptor β1 gene",

abstract = "We report the isolation and characterization of genomic sequences coding for the 5' end of human retinoic acid receptor β1, a fetal RAR isoform which is also expressed in small cell lung cancers. Primer extension analysis revealed a principal transcription start site with a secondary site 23 bp further upstream in both SCLC and fetal tissues. The sequences isolated were CpG-rich between -60 and the 3' end of the first exon but there were no features like a TATA-box or an Inr element. In several important aspects, the human RARβ1 differs from its mouse counterpart. The 5'-UTR is 823 bp long and is encoded by four exons (vs two in the mouse giving a 477 bp UTR). The 5' end of the mouse cDNA has been mapped to a 67 bp CT repeat which is absent in our sequences. We were also unable to find evidence for a human isoform RARβ3 which is widely expressed in mouse tissues.",

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T1 - Structure of the human retinoic acid receptor β1 gene

AU - Toulouse, André

AU - Morin, Johane

AU - Pelletier, Manon

AU - Bradley, W. E.C.

PY - 1996/11/11

Y1 - 1996/11/11

N2 - We report the isolation and characterization of genomic sequences coding for the 5' end of human retinoic acid receptor β1, a fetal RAR isoform which is also expressed in small cell lung cancers. Primer extension analysis revealed a principal transcription start site with a secondary site 23 bp further upstream in both SCLC and fetal tissues. The sequences isolated were CpG-rich between -60 and the 3' end of the first exon but there were no features like a TATA-box or an Inr element. In several important aspects, the human RARβ1 differs from its mouse counterpart. The 5'-UTR is 823 bp long and is encoded by four exons (vs two in the mouse giving a 477 bp UTR). The 5' end of the mouse cDNA has been mapped to a 67 bp CT repeat which is absent in our sequences. We were also unable to find evidence for a human isoform RARβ3 which is widely expressed in mouse tissues.

AB - We report the isolation and characterization of genomic sequences coding for the 5' end of human retinoic acid receptor β1, a fetal RAR isoform which is also expressed in small cell lung cancers. Primer extension analysis revealed a principal transcription start site with a secondary site 23 bp further upstream in both SCLC and fetal tissues. The sequences isolated were CpG-rich between -60 and the 3' end of the first exon but there were no features like a TATA-box or an Inr element. In several important aspects, the human RARβ1 differs from its mouse counterpart. The 5'-UTR is 823 bp long and is encoded by four exons (vs two in the mouse giving a 477 bp UTR). The 5' end of the mouse cDNA has been mapped to a 67 bp CT repeat which is absent in our sequences. We were also unable to find evidence for a human isoform RARβ3 which is widely expressed in mouse tissues.

KW - 5'-Untranslated region

KW - Gene structure

KW - Retinoic acid receptor

UR - https://www.scopus.com/pages/publications/0030580294

U2 - 10.1016/S0167-4781(96)00126-1

DO - 10.1016/S0167-4781(96)00126-1

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JO - Biochimica et Biophysica Acta - Gene Structure and Expression

JF - Biochimica et Biophysica Acta - Gene Structure and Expression

IS - 1-2

ER -

Structure of the human retinoic acid receptor β1 gene

Abstract

Keywords

UN SDGs

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