Survival and Growth in vitro of Paramoeba perurans Populations Cultured Under Different Salinities and Temperatures

Catherine Collins; Malcolm Hall; Mark J. Fordyce; Patricia White

doi:10.1016/j.protis.2018.11.003

Survival and Growth in vitro of Paramoeba perurans Populations Cultured Under Different Salinities and Temperatures

Catherine Collins
, Malcolm Hall
, Mark J. Fordyce
, Patricia White

Marine Scotland

Research output: Contribution to journal › Article › peer-review

Abstract

Growth rates of Paramoeba perurans cultures under different temperature and salinity conditions were investigated in vitro over a 15 day period. Optimal population growth, under the experimental conditions, was observed at 15 °C and a salinity of 35‰, with amoebae populations doubling every 14 h. Positive P. perurans populations growth was observed at 15 °C between salinities of above 20‰ and 50‰, and at 8 °C, 11 °C and 18 °C at salinities between 25‰ and 50‰, 50‰ being the maximum salinity tested. Amoebae numbers were sustained at 4 °C. Therefore, lower temperature and salinity thresholds for P. perurans population growth lie between 4 to 8 °C, and salinities of 20 to 25‰, respectively. Upper limits were not determined in this study. The populations remained relatively stable at 4 °C and 2 °C at permissive salinities with respect to numbers of viable amoebae over the 15 day exposure period.

Original language	English
Pages (from-to)	153-167
Number of pages	15
Journal	Protist
Volume	170
Issue number	2
DOIs	https://doi.org/10.1016/j.protis.2018.11.003
Publication status	Published - Apr 2019
Externally published	Yes

Keywords

in vitro
Paramoeba perurans
population growth
salinity
temperature.

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10.1016/j.protis.2018.11.003

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@article{f6f38a9cb3b14192a830e3cbc6189dd9,

title = "Survival and Growth in vitro of Paramoeba perurans Populations Cultured Under Different Salinities and Temperatures",

abstract = "Growth rates of Paramoeba perurans cultures under different temperature and salinity conditions were investigated in vitro over a 15 day period. Optimal population growth, under the experimental conditions, was observed at 15 °C and a salinity of 35‰, with amoebae populations doubling every 14 h. Positive P. perurans populations growth was observed at 15 °C between salinities of above 20‰ and 50‰, and at 8 °C, 11 °C and 18 °C at salinities between 25‰ and 50‰, 50‰ being the maximum salinity tested. Amoebae numbers were sustained at 4 °C. Therefore, lower temperature and salinity thresholds for P. perurans population growth lie between 4 to 8 °C, and salinities of 20 to 25‰, respectively. Upper limits were not determined in this study. The populations remained relatively stable at 4 °C and 2 °C at permissive salinities with respect to numbers of viable amoebae over the 15 day exposure period.",

keywords = "in vitro, Paramoeba perurans, population growth, salinity, temperature.",

author = "Catherine Collins and Malcolm Hall and Fordyce, \{Mark J.\} and Patricia White",

note = "Publisher Copyright: {\textcopyright} 2018",

year = "2019",

month = apr,

doi = "10.1016/j.protis.2018.11.003",

language = "English",

volume = "170",

pages = "153--167",

journal = "Protist",

issn = "1434-4610",

publisher = "Elsevier GmbH",

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TY - JOUR

T1 - Survival and Growth in vitro of Paramoeba perurans Populations Cultured Under Different Salinities and Temperatures

AU - Collins, Catherine

AU - Hall, Malcolm

AU - Fordyce, Mark J.

AU - White, Patricia

PY - 2019/4

Y1 - 2019/4

N2 - Growth rates of Paramoeba perurans cultures under different temperature and salinity conditions were investigated in vitro over a 15 day period. Optimal population growth, under the experimental conditions, was observed at 15 °C and a salinity of 35‰, with amoebae populations doubling every 14 h. Positive P. perurans populations growth was observed at 15 °C between salinities of above 20‰ and 50‰, and at 8 °C, 11 °C and 18 °C at salinities between 25‰ and 50‰, 50‰ being the maximum salinity tested. Amoebae numbers were sustained at 4 °C. Therefore, lower temperature and salinity thresholds for P. perurans population growth lie between 4 to 8 °C, and salinities of 20 to 25‰, respectively. Upper limits were not determined in this study. The populations remained relatively stable at 4 °C and 2 °C at permissive salinities with respect to numbers of viable amoebae over the 15 day exposure period.

AB - Growth rates of Paramoeba perurans cultures under different temperature and salinity conditions were investigated in vitro over a 15 day period. Optimal population growth, under the experimental conditions, was observed at 15 °C and a salinity of 35‰, with amoebae populations doubling every 14 h. Positive P. perurans populations growth was observed at 15 °C between salinities of above 20‰ and 50‰, and at 8 °C, 11 °C and 18 °C at salinities between 25‰ and 50‰, 50‰ being the maximum salinity tested. Amoebae numbers were sustained at 4 °C. Therefore, lower temperature and salinity thresholds for P. perurans population growth lie between 4 to 8 °C, and salinities of 20 to 25‰, respectively. Upper limits were not determined in this study. The populations remained relatively stable at 4 °C and 2 °C at permissive salinities with respect to numbers of viable amoebae over the 15 day exposure period.

KW - in vitro

KW - Paramoeba perurans

KW - population growth

KW - salinity

KW - temperature.

UR - https://www.scopus.com/pages/publications/85065106226

U2 - 10.1016/j.protis.2018.11.003

DO - 10.1016/j.protis.2018.11.003

M3 - Article

C2 - 31071676

AN - SCOPUS:85065106226

SN - 1434-4610

VL - 170

SP - 153

EP - 167

JO - Protist

JF - Protist

IS - 2

ER -

Survival and Growth in vitro of Paramoeba perurans Populations Cultured Under Different Salinities and Temperatures

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Keywords

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