The activity and stability of wheat nitrate reductase in vitro

Peter W. Jones; Máire ní Mhuimhneacháin

doi:10.1016/S0031-9422(00)80733-5

The activity and stability of wheat nitrate reductase in vitro

Peter W. Jones
, Máire ní Mhuimhneacháin

University College Cork

Research output: Contribution to journal › Article › peer-review

Abstract

The activity and decay characteristics of nitrate reductase from wheat (Triticum aestivum) were studied in crude, partially-purified and highly-purified preparations. The decay of nitrate reductase activity in crude extracts was due to spontaneous dissociation of the enzyme and to the effects of two decay factors, one present in the 0-30% and the other in the 50-70% saturated (NH₄)₂SO₄ fraction of a crude extract. Low rates of factor-mediated NR decay in vitro were associated with high levels of NR activity in vivo.

Original language	English
Pages (from-to)	385-392
Number of pages	8
Journal	Phytochemistry
Volume	24
Issue number	3
DOIs	https://doi.org/10.1016/S0031-9422(00)80733-5
Publication status	Published - 1985

Keywords

Gramineae
nitrate reductase
stability.
Triticum aestivum
wheat

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10.1016/S0031-9422(00)80733-5

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abstract = "The activity and decay characteristics of nitrate reductase from wheat (Triticum aestivum) were studied in crude, partially-purified and highly-purified preparations. The decay of nitrate reductase activity in crude extracts was due to spontaneous dissociation of the enzyme and to the effects of two decay factors, one present in the 0-30\% and the other in the 50-70\% saturated (NH4)2SO4 fraction of a crude extract. Low rates of factor-mediated NR decay in vitro were associated with high levels of NR activity in vivo.",

keywords = "Gramineae, nitrate reductase, stability., Triticum aestivum, wheat",

author = "Jones, \{Peter W.\} and Mhuimhneach{\'a}in, \{M{\'a}ire n{\'i}\}",

year = "1985",

doi = "10.1016/S0031-9422(00)80733-5",

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volume = "24",

pages = "385--392",

journal = "Phytochemistry",

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T1 - The activity and stability of wheat nitrate reductase in vitro

AU - Jones, Peter W.

AU - Mhuimhneacháin, Máire ní

PY - 1985

Y1 - 1985

N2 - The activity and decay characteristics of nitrate reductase from wheat (Triticum aestivum) were studied in crude, partially-purified and highly-purified preparations. The decay of nitrate reductase activity in crude extracts was due to spontaneous dissociation of the enzyme and to the effects of two decay factors, one present in the 0-30% and the other in the 50-70% saturated (NH4)2SO4 fraction of a crude extract. Low rates of factor-mediated NR decay in vitro were associated with high levels of NR activity in vivo.

AB - The activity and decay characteristics of nitrate reductase from wheat (Triticum aestivum) were studied in crude, partially-purified and highly-purified preparations. The decay of nitrate reductase activity in crude extracts was due to spontaneous dissociation of the enzyme and to the effects of two decay factors, one present in the 0-30% and the other in the 50-70% saturated (NH4)2SO4 fraction of a crude extract. Low rates of factor-mediated NR decay in vitro were associated with high levels of NR activity in vivo.

KW - Gramineae

KW - nitrate reductase

KW - stability.

KW - Triticum aestivum

KW - wheat

UR - https://www.scopus.com/pages/publications/0343191542

U2 - 10.1016/S0031-9422(00)80733-5

DO - 10.1016/S0031-9422(00)80733-5

M3 - Article

AN - SCOPUS:0343191542

SN - 0031-9422

VL - 24

SP - 385

EP - 392

JO - Phytochemistry

JF - Phytochemistry

IS - 3

ER -

The activity and stability of wheat nitrate reductase in vitro

Abstract

Keywords

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