The development of an amperometric microbial biosensor using Acetobacter pasteurianus for lactic acid

J. H.T. Luong; A. Mulchandani; C. A. Groom

doi:10.1016/0168-1656(89)90068-0

The development of an amperometric microbial biosensor using Acetobacter pasteurianus for lactic acid

J. H.T. Luong
, A. Mulchandani
, C. A. Groom

National Research Council of Canada

Research output: Contribution to journal › Article › peer-review

Abstract

A microbial biosensor, using Acetobacter pasteurianus cells and an oxygen electrode, was developed for the determination of lactic acid. The bacterial cells were retained on a nylon membrane and attached to the surface of the oxygen electrode. In view of response time, stability and sensitivity, the biosensor performed best at 26°C and in pH 6 phthalate buffer containing magnesium sulfate. The activity of the retained cells was stable for approximately 170 h and was regenerable. The biosensor exhibited a hyperbolic response to both D- and L-lactic acid in the range of 10^-4 M to 25 × 10^-3 M. However, in the range 10^-4 M to 15 × 10^-4 M the response was linear. The microbial biosensor was applicable for detecting lactate concentration in yogurt and milk, since it was not sensitive to lactose, sucrose and glucose - three major components of such dairy products.

Original language	English
Pages (from-to)	241-252
Number of pages	12
Journal	Journal of Biotechnology
Volume	10
Issue number	3-4
DOIs	https://doi.org/10.1016/0168-1656(89)90068-0
Publication status	Published - Jun 1989
Externally published	Yes

Keywords

Acetobacter pasteurianus
Amperometric
Biosensor
Lactic acid
Oxygen consumption

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10.1016/0168-1656(89)90068-0

Cite this

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title = "The development of an amperometric microbial biosensor using Acetobacter pasteurianus for lactic acid",

abstract = "A microbial biosensor, using Acetobacter pasteurianus cells and an oxygen electrode, was developed for the determination of lactic acid. The bacterial cells were retained on a nylon membrane and attached to the surface of the oxygen electrode. In view of response time, stability and sensitivity, the biosensor performed best at 26°C and in pH 6 phthalate buffer containing magnesium sulfate. The activity of the retained cells was stable for approximately 170 h and was regenerable. The biosensor exhibited a hyperbolic response to both D- and L-lactic acid in the range of 10-4 M to 25 × 10-3 M. However, in the range 10-4 M to 15 × 10-4 M the response was linear. The microbial biosensor was applicable for detecting lactate concentration in yogurt and milk, since it was not sensitive to lactose, sucrose and glucose - three major components of such dairy products.",

keywords = "Acetobacter pasteurianus, Amperometric, Biosensor, Lactic acid, Oxygen consumption",

author = "Luong, \{J. H.T.\} and A. Mulchandani and Groom, \{C. A.\}",

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doi = "10.1016/0168-1656(89)90068-0",

language = "English",

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journal = "Journal of Biotechnology",

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AU - Luong, J. H.T.

AU - Mulchandani, A.

AU - Groom, C. A.

PY - 1989/6

Y1 - 1989/6

N2 - A microbial biosensor, using Acetobacter pasteurianus cells and an oxygen electrode, was developed for the determination of lactic acid. The bacterial cells were retained on a nylon membrane and attached to the surface of the oxygen electrode. In view of response time, stability and sensitivity, the biosensor performed best at 26°C and in pH 6 phthalate buffer containing magnesium sulfate. The activity of the retained cells was stable for approximately 170 h and was regenerable. The biosensor exhibited a hyperbolic response to both D- and L-lactic acid in the range of 10-4 M to 25 × 10-3 M. However, in the range 10-4 M to 15 × 10-4 M the response was linear. The microbial biosensor was applicable for detecting lactate concentration in yogurt and milk, since it was not sensitive to lactose, sucrose and glucose - three major components of such dairy products.

AB - A microbial biosensor, using Acetobacter pasteurianus cells and an oxygen electrode, was developed for the determination of lactic acid. The bacterial cells were retained on a nylon membrane and attached to the surface of the oxygen electrode. In view of response time, stability and sensitivity, the biosensor performed best at 26°C and in pH 6 phthalate buffer containing magnesium sulfate. The activity of the retained cells was stable for approximately 170 h and was regenerable. The biosensor exhibited a hyperbolic response to both D- and L-lactic acid in the range of 10-4 M to 25 × 10-3 M. However, in the range 10-4 M to 15 × 10-4 M the response was linear. The microbial biosensor was applicable for detecting lactate concentration in yogurt and milk, since it was not sensitive to lactose, sucrose and glucose - three major components of such dairy products.

KW - Acetobacter pasteurianus

KW - Amperometric

KW - Biosensor

KW - Lactic acid

KW - Oxygen consumption

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DO - 10.1016/0168-1656(89)90068-0

M3 - Article

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VL - 10

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EP - 252

JO - Journal of Biotechnology

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IS - 3-4

ER -

The development of an amperometric microbial biosensor using Acetobacter pasteurianus for lactic acid

Abstract

Keywords

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