TY - JOUR
T1 - The impact of storage conditions upon gentamicin coated antimicrobial implants
AU - Mullins, Nicholas D.
AU - Deadman, Benjamin J.
AU - Moynihan, Humphrey A.
AU - McCarthy, Florence O.
AU - Lawrence, Simon E.
AU - Thompson, Jonathan
AU - Maguire, Anita R.
N1 - Publisher Copyright:
© 2016 Xi'an Jiaotong University
PY - 2016/12/1
Y1 - 2016/12/1
N2 - A systematic approach was developed to investigate the stability of gentamicin sulfate (GS) and GS/poly (lactic-co-glycolic acid) (PLGA) coatings on hydroxyapatite surfaces. The influence of environmental factors (light, humidity, oxidation and heat) upon degradation of the drug in the coatings was investigated using liquid chromatography with evaporative light scattering detection and mass spectrometry. GS coated rods were found to be stable across the range of environments assessed, with only an oxidizing atmosphere resulting in significant changes to the gentamicin composition. In contrast, rods coated with GS/PLGA were more sensitive to storage conditions with compositional changes being detected after storage at 60 °C, 75% relative humidity or exposure to light. The effect of γ-irradiation on the coated rods was also investigated and found to have no significant effect. Finally, liquid chromatography–mass spectrometry analysis revealed that known gentamines C1, C1a and C2 were the major degradants formed. Forced degradation of gentamicin coatings did not produce any unexpected degradants or impurities.
AB - A systematic approach was developed to investigate the stability of gentamicin sulfate (GS) and GS/poly (lactic-co-glycolic acid) (PLGA) coatings on hydroxyapatite surfaces. The influence of environmental factors (light, humidity, oxidation and heat) upon degradation of the drug in the coatings was investigated using liquid chromatography with evaporative light scattering detection and mass spectrometry. GS coated rods were found to be stable across the range of environments assessed, with only an oxidizing atmosphere resulting in significant changes to the gentamicin composition. In contrast, rods coated with GS/PLGA were more sensitive to storage conditions with compositional changes being detected after storage at 60 °C, 75% relative humidity or exposure to light. The effect of γ-irradiation on the coated rods was also investigated and found to have no significant effect. Finally, liquid chromatography–mass spectrometry analysis revealed that known gentamines C1, C1a and C2 were the major degradants formed. Forced degradation of gentamicin coatings did not produce any unexpected degradants or impurities.
KW - Coatings
KW - Gentamicin
KW - LC–ELSD
KW - Storage stability testing
UR - https://www.scopus.com/pages/publications/84994693956
U2 - 10.1016/j.jpha.2016.05.002
DO - 10.1016/j.jpha.2016.05.002
M3 - Article
AN - SCOPUS:84994693956
SN - 2095-1779
VL - 6
SP - 374
EP - 381
JO - Journal of Pharmaceutical Analysis
JF - Journal of Pharmaceutical Analysis
IS - 6
ER -