TY - JOUR
T1 - Three new Escherichia coli phages from the human gut show promising potential for phage therapy
AU - Dalmasso, Marion
AU - Strain, Ronan
AU - Neve, Horst
AU - Franz, Charles M.A.P.
AU - Cousin, Fabien J.
AU - Ross, R. Paul
AU - Hill, Colin
N1 - Publisher Copyright:
© 2016 Dalmasso et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PY - 2016/6/1
Y1 - 2016/6/1
N2 - With the emergence of multi-drug resistant bacteria the use of bacteriophages (phages) is gaining renewed interest as promising anti-microbial agents. The aim of this study was to isolate and characterize phages from human fecal samples. Three new coliphages, φAPCEc01, φAPCEc02 and φAPCEc03, were isolated. Their phenotypic and genomic characteristics, and lytic activity against biofilm, and in combination with ciprofloxacin, were investigated. All three phages reduced the growth of E. coli strain DPC6051 at multiplicity of infection (MOI) between 10-3 and 105. A cocktail of all three phages completely inhibited the growth of E. coli. The phage cocktail also reduced biofilm formation and prevented the emergence of phage-resistant mutants which occurred with single phage. When combined with ciprofloxacin, phage alone or in cocktail inhibited the growth of E. coli and prevented the emergence of resistant mutants. These three new phages are promising biocontrol agents for E. coli infections.
AB - With the emergence of multi-drug resistant bacteria the use of bacteriophages (phages) is gaining renewed interest as promising anti-microbial agents. The aim of this study was to isolate and characterize phages from human fecal samples. Three new coliphages, φAPCEc01, φAPCEc02 and φAPCEc03, were isolated. Their phenotypic and genomic characteristics, and lytic activity against biofilm, and in combination with ciprofloxacin, were investigated. All three phages reduced the growth of E. coli strain DPC6051 at multiplicity of infection (MOI) between 10-3 and 105. A cocktail of all three phages completely inhibited the growth of E. coli. The phage cocktail also reduced biofilm formation and prevented the emergence of phage-resistant mutants which occurred with single phage. When combined with ciprofloxacin, phage alone or in cocktail inhibited the growth of E. coli and prevented the emergence of resistant mutants. These three new phages are promising biocontrol agents for E. coli infections.
UR - https://www.scopus.com/pages/publications/84976276828
U2 - 10.1371/journal.pone.0156773
DO - 10.1371/journal.pone.0156773
M3 - Article
C2 - 27280590
AN - SCOPUS:84976276828
SN - 1932-6203
VL - 11
JO - PLOS ONE
JF - PLOS ONE
IS - 6
M1 - e0156773
ER -
