Two-photon excited fluorescence microscopy using spectroscopic detection techniques

Research output: Contribution to conferencePaperpeer-review

Abstract

Fluorescence microscopy in combination with spectroscopic detection is a powerful tool for simultaneously studying several independent fluorophores in a sample. In medicine, such a tool is interesting for studies of the localization of various photosensitizers in the development of photodynamic therapy. Two-photon excited fluorescence microscopy of unstained specimens has been combined with spectroscopic detection techniques. This gave a spatial resolution of less than 5 micrometer, a spectral resolution of 200 nm, and a time resolution of 20 ps, all at the same time.

Original languageEnglish
Pagesp 53
Publication statusPublished - 1994
Externally publishedYes
EventProceedings of the 1994 Conference on Lasers and Electro-Optics Europe - Amsterdam, Neth
Duration: 28 Aug 19942 Sep 1994

Conference

ConferenceProceedings of the 1994 Conference on Lasers and Electro-Optics Europe
CityAmsterdam, Neth
Period28/08/942/09/94

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