Abstract
Fluorescence microscopy in combination with spectroscopic detection is a powerful tool for simultaneously studying several independent fluorophores in a sample. In medicine, such a tool is interesting for studies of the localization of various photosensitizers in the development of photodynamic therapy. Two-photon excited fluorescence microscopy of unstained specimens has been combined with spectroscopic detection techniques. This gave a spatial resolution of less than 5 micrometer, a spectral resolution of 200 nm, and a time resolution of 20 ps, all at the same time.
| Original language | English |
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| Pages | p 53 |
| Publication status | Published - 1994 |
| Externally published | Yes |
| Event | Proceedings of the 1994 Conference on Lasers and Electro-Optics Europe - Amsterdam, Neth Duration: 28 Aug 1994 → 2 Sep 1994 |
Conference
| Conference | Proceedings of the 1994 Conference on Lasers and Electro-Optics Europe |
|---|---|
| City | Amsterdam, Neth |
| Period | 28/08/94 → 2/09/94 |
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