Vitamin D-enhanced eggs are protective of wintertime serum 25-hydroxyvitamin D in a randomized controlled trial of adults

Background: Despite numerous animal studies that have illustrated the impact of additional Vitamin D in the diet of hens on the resulting egg Vitamin D content, the effect of the consumption of such eggs on Vitamin D status of healthy individuals has not, to our knowledge, been tested. Objective: We performed a randomized controlled trial (RCT) to investigate the effect of the consumption of Vitamin D-enhanced eggs (produced by feeding hens at the maximum concentration of Vitamin D3 or serum 25-hydroxyVitamin D [25(OH)D3] lawfully allowed in feed) on winter serum 25(OH)D in healthy adults. Design: We conducted an 8-wk winter RCT in adults aged 45-70 y (n = 55) who were stratified into 3 groups and were requested to consume #2 eggs/wk (control group, in which status was expected to decline), 7 Vitamin D3-enhanced eggs/wk, or seven 25(OH)D3- enhanced eggs/wk. Serum 25(OH)D was the primary outcome. Results: Although there was no significant difference (P . 0.1; ANOVA) in the mean preintervention serum 25(OH)D in the 3 groups, it was w7-8 nmol/L lower in the control group than in the 2 groups who consumed Vitamin D-enhanced eggs. With the use of an ANCOVA, in which baseline 25(OH)D was accounted for, Vitamin D3-egg and 25(OH)D3-egg groups were shown to have had significantly higher (P # 0.005) postintervention serum 25(OH)D than in the control group. With the use of a within-group analysis, it was shown that, although serum 25(OH)D in the control group significantly decreased over winter (mean 6 SD: 26.4 6 6.7 nmol/L; P = 0.001), there was no change in the 2 groups who consumed Vitamin D-enhanced eggs (P . 0.1 for both). Conclusion: Weekly consumption of 7 Vitamin D-enhanced eggs has an important impact on winter Vitamin D status in adults. This trial was registered at clinicaltrials.gov as NCT02678364. Am J Clin Nutr 2016;104:629-37.