TY  - JOUR
  - Bahey-El-Din M, Gahan CG
  - 2014
  - January
  - Methods in molecular biology (Clifton, N.J.)
  - Vaccination studies: detection of a Listeria monocytogenes-specific T cell immune response using the ELISPOT technique.
  - Validated
  - ()
  - 1157
  - 263
  - 274
  - During systemic infection by Listeria monocytogenes the host develops a robust T cell-mediated immune response against the major immunodominant antigens of the pathogen. The enzyme-linked immuno-spot (ELISPOT) test is an accurate and reproducible means of measuring the extent of this T cell response. Here we describe a detailed ELISPOT protocol for measuring an epitope-specific CD8+ T cell-mediated immune response in mice vaccinated with low doses of L. monocytogenes. The basic approach can be easily adapted for the analysis of other vaccination regimes and target epitopes.
  - 10.1007/978-1-4939-0703-8_22
DA  - 2014/01
ER  - 

@article{V268806205,
   = {Bahey-El-Din M,  Gahan CG },
   = {2014},
   = {January},
   = {Methods in molecular biology (Clifton, N.J.)},
   = {Vaccination studies: detection of a Listeria monocytogenes-specific T cell immune response using the ELISPOT technique.},
   = {Validated},
   = {()},
   = {1157},
  pages = {263--274},
   = {{During systemic infection by Listeria monocytogenes the host develops a robust T cell-mediated immune response against the major immunodominant antigens of the pathogen. The enzyme-linked immuno-spot (ELISPOT) test is an accurate and reproducible means of measuring the extent of this T cell response. Here we describe a detailed ELISPOT protocol for measuring an epitope-specific CD8+ T cell-mediated immune response in mice vaccinated with low doses of L. monocytogenes. The basic approach can be easily adapted for the analysis of other vaccination regimes and target epitopes.}},
   = {10.1007/978-1-4939-0703-8_22},
  source = {IRIS}
}