IRIS publication 43336783
Role of interleukin (IL-10) in probiotic-mediated immune modulation: an assessment in wild-type and IL-10 knock-out mice
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TY - JOUR - Sheil, B,MacSharry, J,O'Callaghan, L,O'Riordan, A,Waters, A,Morgan, J,Collins, JK,O'Mahony, L,Shanahan, F - 2006 - May - Clinical and Experimental Immunology - Role of interleukin (IL-10) in probiotic-mediated immune modulation: an assessment in wild-type and IL-10 knock-out mice - Validated - () - colitis cytokines probiotics INFLAMMATORY-BOWEL-DISEASE PLACEBO-CONTROLLED TRIAL INTERLEUKIN-10-DEFICIENT MICE ESCHERICHIA-COLI MURINE COLITIS DOUBLE-BLIND MAINTAINING REMISSION ULCERATIVE-COLITIS INTESTINAL INFLAMMATION ENTERIC MICROFLORA - 144 - 273 - 280 - While the impact of Bifidobacterium infantis 35624 and other probiotics on cytokines has been shown in established colitis, the effects of B. infantis consumption in pre-inflammation of interleukin (IL)-10 knock-out (KO) mice and on the wild-type (WT) C57Bl/6 mice have not been well demonstrated. The objective of this study was to examine cytokine responses in mucosal and systemic lymphoid compartments of IL-10 KO mice early in disease and to compare with control WT mice. Mice were fed B. infantis or placebo for 5 weeks and culled prior to the onset of chronic intestinal inflammation (12-14 weeks). The spleen, Peyer's patches and intestinal mucosa were removed and stimulated with various bacterial stimuli. Cytokine levels were measured by enzyme-linked immunosorbent assay. While basal intestinal and systemic cytokine profiles of WT and IL-10 KO mice were similar, transforming growth factor (TGF)-beta was reduced in the spleen of IL-10 KO mice. Following probiotic consumption, interferon (IFN)-gamma was reduced in the Peyer's patch of both WT and IL-10 KO mice. Alterations in IFN-gamma in the Peyer's patches of WT mice (enhancement) versus IL-10 KO (reduction) were observed following in vitro stimulation with salmonella. Differential IL-12p40, CCL2 and CCL5 responses were also observed in IL-10 KO mice and WT mice. The cytokine profile of IL-10 KO mice in early disease was similar to that of WT mice. The most pronounced changes occurred in the Peyer's patch of IL-10 KO mice, suggesting a probiotic mechanism of action independent of IL-10. This study provides a rationale for the use of B. infantis 35624 for the treatment of gastrointestinal inflammation. - DOI 10.1111/j.1365-2249.2006.03051.x DA - 2006/05 ER -
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@article{V43336783, = {Sheil, B and MacSharry, J and O'Callaghan, L and O'Riordan, A and Waters, A and Morgan, J and Collins, JK and O'Mahony, L and Shanahan, F }, = {2006}, = {May}, = {Clinical and Experimental Immunology}, = {Role of interleukin (IL-10) in probiotic-mediated immune modulation: an assessment in wild-type and IL-10 knock-out mice}, = {Validated}, = {()}, = {colitis cytokines probiotics INFLAMMATORY-BOWEL-DISEASE PLACEBO-CONTROLLED TRIAL INTERLEUKIN-10-DEFICIENT MICE ESCHERICHIA-COLI MURINE COLITIS DOUBLE-BLIND MAINTAINING REMISSION ULCERATIVE-COLITIS INTESTINAL INFLAMMATION ENTERIC MICROFLORA}, = {144}, pages = {273--280}, = {{While the impact of Bifidobacterium infantis 35624 and other probiotics on cytokines has been shown in established colitis, the effects of B. infantis consumption in pre-inflammation of interleukin (IL)-10 knock-out (KO) mice and on the wild-type (WT) C57Bl/6 mice have not been well demonstrated. The objective of this study was to examine cytokine responses in mucosal and systemic lymphoid compartments of IL-10 KO mice early in disease and to compare with control WT mice. Mice were fed B. infantis or placebo for 5 weeks and culled prior to the onset of chronic intestinal inflammation (12-14 weeks). The spleen, Peyer's patches and intestinal mucosa were removed and stimulated with various bacterial stimuli. Cytokine levels were measured by enzyme-linked immunosorbent assay. While basal intestinal and systemic cytokine profiles of WT and IL-10 KO mice were similar, transforming growth factor (TGF)-beta was reduced in the spleen of IL-10 KO mice. Following probiotic consumption, interferon (IFN)-gamma was reduced in the Peyer's patch of both WT and IL-10 KO mice. Alterations in IFN-gamma in the Peyer's patches of WT mice (enhancement) versus IL-10 KO (reduction) were observed following in vitro stimulation with salmonella. Differential IL-12p40, CCL2 and CCL5 responses were also observed in IL-10 KO mice and WT mice. The cytokine profile of IL-10 KO mice in early disease was similar to that of WT mice. The most pronounced changes occurred in the Peyer's patch of IL-10 KO mice, suggesting a probiotic mechanism of action independent of IL-10. This study provides a rationale for the use of B. infantis 35624 for the treatment of gastrointestinal inflammation.}}, = {DOI 10.1111/j.1365-2249.2006.03051.x}, source = {IRIS} }
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AUTHORS | Sheil, B,MacSharry, J,O'Callaghan, L,O'Riordan, A,Waters, A,Morgan, J,Collins, JK,O'Mahony, L,Shanahan, F | ||
YEAR | 2006 | ||
MONTH | May | ||
JOURNAL_CODE | Clinical and Experimental Immunology | ||
TITLE | Role of interleukin (IL-10) in probiotic-mediated immune modulation: an assessment in wild-type and IL-10 knock-out mice | ||
STATUS | Validated | ||
TIMES_CITED | () | ||
SEARCH_KEYWORD | colitis cytokines probiotics INFLAMMATORY-BOWEL-DISEASE PLACEBO-CONTROLLED TRIAL INTERLEUKIN-10-DEFICIENT MICE ESCHERICHIA-COLI MURINE COLITIS DOUBLE-BLIND MAINTAINING REMISSION ULCERATIVE-COLITIS INTESTINAL INFLAMMATION ENTERIC MICROFLORA | ||
VOLUME | 144 | ||
ISSUE | |||
START_PAGE | 273 | ||
END_PAGE | 280 | ||
ABSTRACT | While the impact of Bifidobacterium infantis 35624 and other probiotics on cytokines has been shown in established colitis, the effects of B. infantis consumption in pre-inflammation of interleukin (IL)-10 knock-out (KO) mice and on the wild-type (WT) C57Bl/6 mice have not been well demonstrated. The objective of this study was to examine cytokine responses in mucosal and systemic lymphoid compartments of IL-10 KO mice early in disease and to compare with control WT mice. Mice were fed B. infantis or placebo for 5 weeks and culled prior to the onset of chronic intestinal inflammation (12-14 weeks). The spleen, Peyer's patches and intestinal mucosa were removed and stimulated with various bacterial stimuli. Cytokine levels were measured by enzyme-linked immunosorbent assay. While basal intestinal and systemic cytokine profiles of WT and IL-10 KO mice were similar, transforming growth factor (TGF)-beta was reduced in the spleen of IL-10 KO mice. Following probiotic consumption, interferon (IFN)-gamma was reduced in the Peyer's patch of both WT and IL-10 KO mice. Alterations in IFN-gamma in the Peyer's patches of WT mice (enhancement) versus IL-10 KO (reduction) were observed following in vitro stimulation with salmonella. Differential IL-12p40, CCL2 and CCL5 responses were also observed in IL-10 KO mice and WT mice. The cytokine profile of IL-10 KO mice in early disease was similar to that of WT mice. The most pronounced changes occurred in the Peyer's patch of IL-10 KO mice, suggesting a probiotic mechanism of action independent of IL-10. This study provides a rationale for the use of B. infantis 35624 for the treatment of gastrointestinal inflammation. | ||
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DOI_LINK | DOI 10.1111/j.1365-2249.2006.03051.x | ||
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