TY  - JOUR
  - Sheil, B,MacSharry, J,O'Callaghan, L,O'Riordan, A,Waters, A,Morgan, J,Collins, JK,O'Mahony, L,Shanahan, F
  - 2006
  - May
  - Clinical and Experimental Immunology
  - Role of interleukin (IL-10) in probiotic-mediated immune modulation: an assessment in wild-type and IL-10 knock-out mice
  - Validated
  - ()
  - colitis cytokines probiotics INFLAMMATORY-BOWEL-DISEASE PLACEBO-CONTROLLED TRIAL INTERLEUKIN-10-DEFICIENT MICE ESCHERICHIA-COLI MURINE COLITIS DOUBLE-BLIND MAINTAINING REMISSION ULCERATIVE-COLITIS INTESTINAL INFLAMMATION ENTERIC MICROFLORA
  - 144
  - 273
  - 280
  - While the impact of Bifidobacterium infantis 35624 and other probiotics on cytokines has been shown in established colitis, the effects of B. infantis consumption in pre-inflammation of interleukin (IL)-10 knock-out (KO) mice and on the wild-type (WT) C57Bl/6 mice have not been well demonstrated. The objective of this study was to examine cytokine responses in mucosal and systemic lymphoid compartments of IL-10 KO mice early in disease and to compare with control WT mice. Mice were fed B. infantis or placebo for 5 weeks and culled prior to the onset of chronic intestinal inflammation (12-14 weeks). The spleen, Peyer's patches and intestinal mucosa were removed and stimulated with various bacterial stimuli. Cytokine levels were measured by enzyme-linked immunosorbent assay. While basal intestinal and systemic cytokine profiles of WT and IL-10 KO mice were similar, transforming growth factor (TGF)-beta was reduced in the spleen of IL-10 KO mice. Following probiotic consumption, interferon (IFN)-gamma was reduced in the Peyer's patch of both WT and IL-10 KO mice. Alterations in IFN-gamma in the Peyer's patches of WT mice (enhancement) versus IL-10 KO (reduction) were observed following in vitro stimulation with salmonella. Differential IL-12p40, CCL2 and CCL5 responses were also observed in IL-10 KO mice and WT mice. The cytokine profile of IL-10 KO mice in early disease was similar to that of WT mice. The most pronounced changes occurred in the Peyer's patch of IL-10 KO mice, suggesting a probiotic mechanism of action independent of IL-10. This study provides a rationale for the use of B. infantis 35624 for the treatment of gastrointestinal inflammation.
  - DOI 10.1111/j.1365-2249.2006.03051.x
DA  - 2006/05
ER  - 

@article{V43336783,
   = {Sheil,  B and MacSharry,  J and O'Callaghan,  L and O'Riordan,  A and Waters,  A and Morgan,  J and Collins,  JK and O'Mahony,  L and Shanahan,  F },
   = {2006},
   = {May},
   = {Clinical and Experimental Immunology},
   = {Role of interleukin (IL-10) in probiotic-mediated immune modulation: an assessment in wild-type and IL-10 knock-out mice},
   = {Validated},
   = {()},
   = {colitis cytokines probiotics INFLAMMATORY-BOWEL-DISEASE PLACEBO-CONTROLLED TRIAL INTERLEUKIN-10-DEFICIENT MICE ESCHERICHIA-COLI MURINE COLITIS DOUBLE-BLIND MAINTAINING REMISSION ULCERATIVE-COLITIS INTESTINAL INFLAMMATION ENTERIC MICROFLORA},
   = {144},
  pages = {273--280},
   = {{While the impact of Bifidobacterium infantis 35624 and other probiotics on cytokines has been shown in established colitis, the effects of B. infantis consumption in pre-inflammation of interleukin (IL)-10 knock-out (KO) mice and on the wild-type (WT) C57Bl/6 mice have not been well demonstrated. The objective of this study was to examine cytokine responses in mucosal and systemic lymphoid compartments of IL-10 KO mice early in disease and to compare with control WT mice. Mice were fed B. infantis or placebo for 5 weeks and culled prior to the onset of chronic intestinal inflammation (12-14 weeks). The spleen, Peyer's patches and intestinal mucosa were removed and stimulated with various bacterial stimuli. Cytokine levels were measured by enzyme-linked immunosorbent assay. While basal intestinal and systemic cytokine profiles of WT and IL-10 KO mice were similar, transforming growth factor (TGF)-beta was reduced in the spleen of IL-10 KO mice. Following probiotic consumption, interferon (IFN)-gamma was reduced in the Peyer's patch of both WT and IL-10 KO mice. Alterations in IFN-gamma in the Peyer's patches of WT mice (enhancement) versus IL-10 KO (reduction) were observed following in vitro stimulation with salmonella. Differential IL-12p40, CCL2 and CCL5 responses were also observed in IL-10 KO mice and WT mice. The cytokine profile of IL-10 KO mice in early disease was similar to that of WT mice. The most pronounced changes occurred in the Peyer's patch of IL-10 KO mice, suggesting a probiotic mechanism of action independent of IL-10. This study provides a rationale for the use of B. infantis 35624 for the treatment of gastrointestinal inflammation.}},
   = {DOI 10.1111/j.1365-2249.2006.03051.x},
  source = {IRIS}
}